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197 Vitronectin (human) 0.25mg
Part Number 197
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$750.00
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Description
Description
Human vitronectin, lyophilized (VTN; S-protein; Serum spreading factor; Epiboin). Vitronectin and fibronectin are the most studied serum-derived proteins that promote attachment and adhesion of cultured cells. Although fibronectin is more commonly used in cell culture, studies show that vitronectin is more effective substrate for cell adhesion and spreading, especially in the presence of other serum proteins when adsorption (1, 2). Vitronectin improved support of ocular surface stem cells and facilitated corneal regeneration (3). Vitronectin-coated surfaces supported human embryonic stem cells (ES) and induced pluripotent stem cells (iPS) even without feeder cells or Matrigel (4, 5, 6, 7, 8)
Catalog Number 197
Amount 0.25 mg

Source
Human plasma, certified by a supplier to be HIV- and HbsAg-negative

Purity
SDS-PAGE under reducing conditions and visualized by silver staining - 90% purity. Endotoxin content 1.0 EU/ug of the protein by the LAL method

Applications
ELISA, Cell culture. Activity is tested by  adhesion of DU145 human prostate carcinoma cells to vitronectin-coated plates
Format and reconstitution
Lyophilized from a 0.2um filtered solution in PBS and urea. Reconstitute at 0.1 mg/ml in sterile PBS
Storage 6 months at -20C from date of shipment

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE

1. Knox and Griffith (1980) The distribution of cell-spreading activities in sera: a 
quantitative approach. J. Cell Sci. 46:97-112

2. Underwood PA and Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J. Cell Science 93:641-649

3. Echevarria TJ, Chow S, Watson S, Wakefield D, Di Girolamo N. 
(2011) Vitronectin: a matrix support factor for human limbal epithelial progenitor cells. Invest Ophthalmol Vis Sci. 2011 2(11):8138-47

4. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 8(5):424-429

5. Rowland TJ, Miller LM, Blaschke AJ, Doss EL, Bonham AJ, Hikita ST, Johnson LV, Clegg DO. (2010) Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 19(8):1231-40

6. Li J, Bardy J, Yap LY, Chen A, Nurcombe V, Cool SM, Oh SK, Birch WR. (2010) Impact of vitronectin concentration and surface properties on the stable propagation of human embryonic stem cells. Biointerphases. 5(3):FA132-42.

7. Yap LY, Li J, Phang IY, Ong LT, Ow JZ, Goh JC, Nurcombe V, Hobley J, Choo AB, Oh SK, Cool SM, Birch WR. (2011) Defining a threshold surface density of vitronectin for the stable expansion of human embryonic stem cells. Tissue Eng Part C Methods. 17(2):193-207

8. Heydarkhan-Hagvall S, Gluck JM, Delman C, Jung M, Ehsani N, Full S, Shemin RJ. (2012) The effect of vitronectin on the differentiation of embryonic stem cells in a 3D culture system.Biomaterials. 33(7):2032-40

Disclaimer: Studies referenced herein used vitronectin from variety of sources

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