Full length native Apolipoprotein A1 protein purified from human plasma

Source

Human plasma. Donors tested negative for HBsAg, HBc, HIV-I, HIV-II and HCV antibodies

Purity

At least 90% pure, judged by SDS-PAGE

Format

Lyophilized from 0.05 M Na-chloride, 0.01 M Na-carbonate, pH 10.0

Purification

Ultracentrifugation in density gradient. Delipidation with diethyl ether and ethanol. Gel filtration on Sephacryl S-300

Applications

Apolipoprotein A1 standard. Antigen for antibody production. Coating material for cell culture studies. ELISA

Reconstitution

Sterile pure water or PBS

Storage

Storage of lyophilized product 2 years at -20°C. Storage of reconstituted product 1 month at +4°C, 1 year at -20°C or lower. Avoid multiple freeze-thaw cycles