Apolipoprotein B protein (low density lipoproteins LDL) purified from human plasma

Source

Human plasma. Donors tested negative for HBsAg, HBc, HIV-I, HIV-II and HCV antibodies

Purity

At least 90% pure, judged by SDS-PAGE. Band at 550 kDa

Format

Solution containing 50 mM Na2CO3, pH 10, 50 mM NaCl and deozycholate. Non-sterile

Purification

Ultracentrifugation in density gradient. Delipidation with diethyl ether and ethanol. Gel filtration on Sephacryl S-300

Applications

Apolipoprotein B standard. Antigen for antibody production. Coating material for cell culture studies. ELISA

Reconstitution

Sterile pure water or PBS

Storage

Storage of reconstituted product 1 month at +4°C, 1 year at -20°C or lower. Avoid multiple freeze-thaw cycles