Native human collagen type I protein purified from human placenta

Source

Human placenta. Negative for HBsAg, HCV, HIV1 and HIV2

Purity

Human collagen type I at least 90%, human collagen type III below 10%. Other human collagens and non-collagen proteins below 1%. Purity is controlled by SDS-PAGE and by ELISA with anti-collagen type-specific antibodies

Format

Lyophilized

Purification

Partial pepsin digestion in acidic conditions, differential salt precipitation and DEAE ion-exchange chromatography

Applications

Human collagen type I standard. Antigen for antibody production. Coating material for cell culture studies

Reconstitution

Use 0.1 M acetic acid, pH 3.0

Storage

Shipping at ambient temperature. Storage 2 years at -20°C or lower

Publications: Brokopp CE, Schoenauer R, Richards P, Bauer S, Lohmann C, Emmert MY, Weber B, Winnik S, Aikawa E, Graves K, Genoni M, Vogt P, Lüscher TF, Renner C, Hoerstrup SP, Matter CM. (2011) Fibroblast activation protein is induced by inflammation and degrades type I collagen in thin-cap fibroatheromata. Eur Heart J. 2011 Nov;32(21):2713-22