Native bovine collagen type I and III protein purified from calf skin

Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Bovine collagen type I – 45%, bovine collagen type III – 45%, bovine collagen other types <10%, non-collagen proteins <0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C. Do not freeze reconstituted collagen