Native canine collagen type I and III

Source

Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Canine collagen I and III – 98%, canine collagen (other types) <1%, non-collagen proteins <0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 2.5 . Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C