Native porcine collagen type I and III purified from pig's tissue

Source

Porcine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Porcine collagens: type I – 45%, type III – 45%, type IV – 10%, type V below 1%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 3.0. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C