Native bovine collagen type III isolated from calf skin

Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type III was purified by using differential salt precipitation

Purity

Bovine collagen type III 90%. Other bovine collagen types 10%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Bovine collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC