Native rat collagen type III

Source

Newborn rats. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type III was purified by using differential salt precipitation

Purity

Rat collagen type III 60%. Other rat collagens 40%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Rat collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC