Mixture of proteolytic enzymes isolated from King Crab (Paralithodes Camtschatica) hepatopancreases

Source

King Crab (Paralithodes Camtschatica) hepatopancreases

Purity

Five bands in the range 18-34 kDa

Format

Lyophilized

Purification

Acetone extraction. Affinity and ion-exchange chromatography

Applications

Isolation of animal cells from tissues and cultureware. Use at pH 6-9, 4-40°C, 1-10mM CaCl2. Inhibition by DFP, PMSF and EDTA

Reconstitution

Dissolve in buffer containing 1-10mM CaCl2 at pH 6-9

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower