Fibronectin purified from human plasma (lyophilized)

Source

Human plasma. Donors tested negative for HBsAg, HCV, HIV1 and HIV2 antibodies

Purification

Affinity chromatography on gelatine agarose and heparin agarose

Format

Lyophilized from 2.5 ml of buffer (150 mM NaCl, 10 mM sodium phosphate pH 7.5). Non-sterile.

Reconstitution

Add 5 ml of warm (37°C) PBS . Incubate at 37°C for 3 hours, occasionally inverting the vial. Do not vortex. Reconstituted solution contains 5 mg of soluble fibronectin and a minor quantity of insoluble fibronectin aggregate. Filter slowly through 0.2 µm membrane filter. Concentration 1 mg/ml of fibronectin corresponds to A280 - 1.3

Storage

Lyophilized protein: shipping at ambient temperature, storage at –20°C or lower. Lyophilized product is moisture-sensitive. Reconstituted and sterile-filtered product can be stored for 1 month at +2...8°C