Human integrin aVb5, affinity purified, lyophilized (ITGAV, MSK8, CD51, VNRA)

Source

Human placenta. Product was tested and found negative for HIV and Hepatitis B

Purity

SDS-PAGE with silver staining at least 95%. Two major bands corresponding to aV (145 kDa) and b5 (90 kDa) subunits (non-reduced conditions).

Format

Lyophilized from solution containing: 0.26 mg/ml aVb5, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl₂, 0.2% Triton X-100

Purification

Affinity chromatography with immobilized monoclonal anti-aVb5 antibodies. A tissue detergent extract, which was applied to the column was prepared as described in (Belkin et al. 1990, J. Cell. Biol. 111:2159-2170)

Applications

Standard for electrophoresis, immunobloting and ligand binding

Reconstitution

Water. Keep reconstituted protein at -70°C. Avoid repeated freeze-thaw cycles

Storage

Lyophilized product: shipping at ambient temperature, long-term storage at -20°C. Reconstituted protein: 1 year at -70°C