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126 Integrin a1b1 (human) 5 ug
Part Number 126
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$99.00
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Description

Human integrin a1b1, affinity purified, lyophilized (VLA-1, CD49a/CD29, ITGA1)

Source

Human smooth muscles. Product was tested and found negative for HIV and Hepatitis B

Purity

SDS-PAGE with silver staining at least 95%. Two major bands corresponding to a1 (195 kDa) and b1 (120 kDa) subunits (non-reduced conditions). A minor band (210 kDa) corresponds to b1 subunit disulphide dimer.

Format

Lyophilized from solution containing: 0.28 mg/ml a1b1, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100

Purification

Affinity chromatography. A tissue detergent extract, which was applied to the column was prepared as described in (Belkin et al. 1990, J. Cell. Biol. 111:2159-2170)

Applications

Integrin a1b1 interacts with collagen I, IV and laminin in ELISA. This format of a1b1 is appropriate for functional evaluation.

Reconstitution

Water. Keep reconstituted protein at -70C. Avoid repeated freeze-thaw cycles

Storage

Lyophilized product: shipping at ambient temperature, long-term storage at -20C. Reconstituted protein: 1 year at -70C

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