| Human integrin aVb3,
affinity purified, lyophilized (vitronectin receptor, CD51/CD61,
ITGAV, DKFZp686A08142, MSK8, VNRA) Source
Human placenta. Product
was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE
with silver staining at least 95%. Two major bands corresponding to
aV (145 kDa) and b3
(92 kDa) subunits (non-reduced conditions). A minor band (170 kDa)
corresponds to b3 subunit
disulphide dimer.
Format
Lyophilized from solution
containing: 0.21 mg/ml aVb3,
20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2%
Triton X-100
Purification
Affinity
chromatography with immobilized monoclonal anti-aVb3
antibodies. A tissue detergent extract, which was applied to the
column was prepared as described in (Belkin et al. 1990, J. Cell.
Biol. 111:2159-2170)
Applications
Integrin aVb3
interacts with vitronectin, von Willebrand factor, fibronectin and
fibrinogen in RGD dependent ELISA test. This format of aVb3
is appropriate for functional evaluation.
- Standard for
electrophoresis, immunobloting and ligand binding
- In vitro binding
experiments with metalloproteinase MMP-2
Reconstitution
Water. Keep reconstituted
protein at -70°C. Avoid
repeated freeze-thaw cycles
Storage
Lyophilized
product: shipping at ambient temperature, long-term storage at -20°C.
Reconstituted protein: 1 year at -70°C |
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