Monoclonal antibody anti-human CD36 (Platelet GPIV, Thrombospondin receptor) (clone VM58, IgG1 kappa fraction)

Specificity

The monoclonal antibody is directed against the CD36 antigen (GP IV, GP IIIa, Thrombospondin receptor) (90 kDa), which is expressed on human platelets. Antibody reacts with platelets, monocytes, macrophages, erythroblasts and weakly with B-cells. In immunohistology the antibody reacts with some endothelial cells, adypocytes and the granular layer of the skin. Antibody induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets

Preparation

Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution

Format

Purified and lyophilized from PBS with sodium azide

Applications

Functional studies. Indirect immunofluorescence. Innumohistochemistry.

For fluorescence technique on cells fixation with 1% paraformaldehyde for 5 minutes is advised. Use for WB is not recommended

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower