Monoclonal mouse (murine) antibody (IgG1 fraction) anti-human Apolipoprotein B, clone 12G10

Specificity

Specific against T4 fragment (res. 1-1297), the N-terminal thrombolytic peptide of human Apolipoprotein B (LDL). No reactivity to other human plasma proteins.

Preparation

Precipitation by sodium sulphate and anion-exchange chromatography (DEAE-cellulose) with gradient elution

Format

Purified and lyophilized. Contains mannitol, dextran and salts

Applications and recommended dilutions

Detection of Apolipoprotein B (LDL) in human plasma or serum

Extraction of Apo-B containing lipoproteins from human plasma

ELISA (x1000), Immunohistology (x100)

Reconstitution

Use 1 ml sterile pure water, aliquote and store at -20°C or lower.

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower.