Specificity

Human Apolipoprotein E (all alleles). Reacts with LDL and VLDL. No cross-reactivity to other human plasma proteins

Preparation

Ammonium sulphate precipitation. DEAE-cellulose chromatography with gradient elution

Format

Lyophilised from solution containing 0.5 mg antibody, 1% mannitol, 0.15 M sodium chloride, 0.01 M sodium phosphate

Applications

Western blotting, ELISA (x1000), Immunohistochemistry (x100)

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower

References

Huter O, Wolf HJ, Schnetzer A, Pfaller K (1997) Lipoprotein lipase, LDL receptors and apo-lipoproteins in human fetal membranes at term. Placenta 18(8):707-715