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Home > antigen > Apolipoprotein E
141 Mouse anti-human Apolipoprotein E monoclonal antibody, clone 3D12 1 ml
Part Number 141
Price
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$195.00
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1 $195.00
2 - 4 $156.00
5+ $136.50
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Description
Monoclonal antibody (clone 3D12, IgG1 fraction) anti-human Apolipoprotein E (APOE)

Specificity

Human Apolipoprotein E (all alleles). Reacts with LDL and VLDL. No cross-reactivity to other human plasma proteins

Preparation

Ammonium sulphate precipitation. DEAE-cellulose chromatography with gradient elution

Format

Lyophilised from solution containing 0.5 mg antibody, 1% mannitol, 0.15 M sodium chloride, 0.01 M sodium phosphate

Applications

Western blotting, ELISA (x1000), Immunohistochemistry (x100), IHC 1:25 (after deparaffinization, rehydration and treatment with pronase E ) (1)

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20C or lower

References

1) Klaver CCW, Kliffen M, van Duijn CM, Hofman A, Cruts M, Grobbee DE, van Broeckhoven C, de Jong PTVM (1998)  Genetic Association of Apolipoprotein E with Age-Related Macular Degeneration. Am. J. Hum. Genet. 63:200206

2) Huter O, Wolf HJ, Schnetzer A, Pfaller K (1997) Lipoprotein lipase, LDL receptors and apo-lipoproteins in human fetal membranes at term. Placenta 18(8):707-715

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