Monoclonal antibody anti-human CD61 (integrin beta 3, ß-integrin, Platelet GPIIIa, VNR ß-chain 3) (IgG1 kappa, clone CRC54)

Specificity

Reacts with platelet glycoprotein gpIIIa/VNR ß chain. This glycoprotein is the common ß chain (ß3 Integrin) of the cytoadhesins, gpIIbIIIa and VNR. Reacts with platelets and megakaryocytes. The antibody reacts preferentially with the activated/occupied form of platelet GPIIb-IIIa (ligand-induced binding

site or LIBS epitope) and dissociated form of GPIIIa. CRC54-epitope is located within the N-terminal region of GPIIIa (first 100 aminoacids). CRC54 does not react with platelets of patients with Glanzmann thrombastenia.

Preparation

Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution

Format

Purified and lyophilized. Contains azide and PBS

Applications and recommended dilutions

Immunoassay, Immunofluorescence, FACS, IHC (frozen)

Antibody can be used for:

-Determination of thrombocytes in blood.

-Identification of megakaryocytes and -blasts in tissue.

-Immunoblotting under reduced and nonreduced conditions.

-Detection of conformational changes of GPIIb-IIIa on activated platelets.

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower