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Home > antigen > IgA
173 Sheep anti-rabbit IgG, IgM, IgA polyclonal antibody FITC 1.2 mg
Part Number 173
Price
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$195.00
Availability:
dispatched within 2 days
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2+ $156.00
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Description

Polyclonal sheep antibody anti-rabbit immunoglobulins IgG, IgM, IgA conjugated to FITC

Specificity

Binds rabbit immunoglobulins IgG, IgM, IgA. Affinity to human IgM, IgG, IgA as measured by ELISA below 3%

Preparation

Purification by affinity chromatography using antigen coupled to sorbent. Antibody concentration was determined by extinction coefficient prior to conjugation to fluorescein isothiocyanate (FITC). Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody

Format

Solution 1.2 mg/mL antibody, 0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA

Applications and recommended dilutions

Western blotting, ELISA, Immunohistology (1:200), Cell staining, Cell sorting, Electron microscopy

Storage

Shipping at ambient temperature. Storage at 20C for 2 years

Citations 

1. Ehlicke, F., Freimark, D., Heil, B., Dorresteijn, A., Czermak, P. (2010). Intervertebral disc regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC). International Journal of Artificial Organs, 33 (4), 244-252.

2. Lee E, Choi J, Lee HS (2017) Palmitate induces mitochondrial superoxide generation and activates AMPK in podocytes. J. Cellular Physiology 232:12:3209-3217

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