Polyclonal goat antibody anti-human IgG conjugated to FITC (affinity purified and cross-adsorbed)

Specificity

Human immunoglobulin IgG. No cross reactivity to human IgM, IgA or other human plasma proteins

Preparation

Purification by affinity chromatography using immobilized human IgG. Cross-adsorption on immobilized human IgM and IgA. Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody

Format

Antibody solution 1.2 mg/ml in the buffer (0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA)

Applications and recommended dilutions

Western blotting, ELISA, Immunohistology (1:40), Cell staining, Cell sorting, Electron microscopy

Storage

Shipping at ambient temperature. Storage at –20°C for 2 years