Polyclonal rabbit antibody anti-human IgM conjugated to horseradish peroxidase (HRP) (cross-adsorbed and affinity purified)

Specificity

Human immunoglobulin IgM. No reactivity against human IgG, IgA, other serum proteins

Preparation

Purification by affinity chromatography using immobilized IgM. Cross-adsorption against human IgG, IgA and other serum proteins. Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase.

Format

Solution 1.2 mg/mL antibody, 50% glycerol, 0.1M NaCl, 0.05M sodium phosphate pH 7.5, 5 mg/ml BSA

Applications and recommended dilutions

ELISA (x10000), Immunohistology, Blotting. Dilution should be adjusted to the appropriate task

Storage

Shipping at ambient temperature. Storage for 1 year at -20°C or lower