Polyclonal rabbit antibody to human collagen type I (affinity purified and cross-adsorbed) COL1A1

Specificity

Binds native 3D epitopes of Human Collagen type I. No cross-reactivity to human plasma proteins and human collagen types II, III, IV and V

Preparation

Affinity purification on immobilized collagen type I with cross-absorption to immobilised human blood plasma proteins and human collagen type II, III, IV and V

Format

Purified and lyophilized. Contains mannitol, dextran and salts

Applications and recommended dilutions

Detection of human collagen type I by ELISA (x3000), Histochemistry (frozen sections) (x20). Immunostaining of ECM in cell culture. Not tested for use on paraffin section.

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower