Specificity

Binds native 3D epitopes of rat collagen type I. Cross-reactivity to denatured rat collagen type I 50%.

Preparation

Affinity purification on immobilized collagen type I with cross-absorption to immobilised rat collagen type III

Format

Purified and lyophilized. Contains mannitol, dextran and salts

Applications and recommended dilutions

Detection of rat collagen type I by sandwich ELISA (x1000), Histochemistry (frozen sections) (x20). Immunostaining of ECM in rat cell culture.

Reconstitution

Use 0.5 or 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower