Porcine atelocollagen type I, sterile and lyophilised, 30 mg — a gel-forming 3D-culture and scaffold matrix. Extended pepsin digestion removes the non-helical N- and C-terminal telopeptides, the primary source of cross-species immunogenicity in collagen scaffolds, while the triple-helical molecule retains native conformation and self-assembly: neutralised at 37 °C it polymerises into a translucent, mechanically stable fibrillar gel for embedding cells, spheroids or explants. Supplied sterile — no further sterilisation required. The low-antigenicity alternative to native porcine type I cat. 882 and to the partial-pepsin-digested I/III blends cat. 223 and cat. 785.
SPECIFICATIONS
Catalogue number: 130 | Source: Porcine skin / tendon
Composition: Collagen type I (atelocollagen, telopeptide-depleted)
Purification: Extended pepsin digestion under acidic conditions, neutral-salt precipitation, dialysis, sterile filtration of the soluble pre-form, lyophilisation
Format: Lyophilised, sterile | Package size: 30 mg
Suitable for: 3D collagen gel / hydrogel formation, cell encapsulation, organoid culture, scaffold coating, immunoassay reference
STORAGE / RECONSTITUTION
Dissolve cold in 0.01–0.02 M acetic acid at 4 °C overnight. Do not vortex or heat above 4 °C before neutralisation. Ships at ambient temperature. Store lyophilised at −20 °C; store the reconstituted acidic solution up to 1 month at +4 °C.
APPLICATIONS
- 3D cell culture and organoid scaffolds — gel-forming porcine ECM with low cross-species immunogenicity.
- Hydrogel formulations — standalone or blended with type III collagen, hyaluronic acid, fibrin or PEG.
- Cell encapsulation and 3D migration / invasion assays — embedded tumour-spheroid, fibroblast and stem-cell models.
- Coating substrate for 2D culture of porcine, primary mammalian and induced pluripotent stem cells.
- Reference / positive-control material for porcine collagen identification by qPCR and ELISA in halal authentication, food-fraud testing and species-of-origin verification.
- Antigen for raising anti-porcine type I collagen antibodies.
- Tissue-engineering scaffolds, decellularised-matrix recellularisation and bioprinting workflows where animal-origin traceability is required; the bioprinting-grade counterpart is cat. 144.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Low-immunogenicity 3D matrices — telopeptide-depleted collagen for constructs intended for in-vivo or pre-clinical grafting.
- Halal / food-authentication testing — certified porcine positive control for qPCR and ELISA species detection.
- Tumour-invasion and spheroid models — reproducible fibrillar gels with defined stiffness.
- Regenerative medicine and wound-healing scaffolds — porcine ECM as a dermal-substitute backbone.
- Stem-cell and organoid culture — sterile, ready-to-reconstitute matrix without additional processing.
REFERENCES
Basuki C.D.P., Zainal R., Abdullah Sani M.S. (2025) Evaluating PCR and ELISA for porcine detection in collagen-based products for halal authentication. HALALSPHERE 6(2): 8–18. Powdered porcine type I collagen (cat. 130) was used as the certified positive control for the qPCR standard curve and for ELISA plate validation in a two-method comparison on nine commercial collagen-based consumer products.
