Bovine collagen type IV (atelocollagen) purified from placenta, sterile and lyophilised, 1 mg — the basement-membrane collagen, supplied as a coating substrate for cell culture and as an immunoassay antigen. Mild pepsin treatment removes the telopeptides, lowering cross-species antigenicity while preserving the type IV network-forming domain structure. Use as the bovine counterpart of human collagen type IV cat. 425; the bovine fibrillar collagens cat. 437 (type I atelocollagen), cat. 576 (type III atelocollagen) and cat. 184 (type V atelocollagen) complete the bovine ECM panel.
SPECIFICATIONS
Catalogue number: 148 | Source: Bovine placenta
Composition: Collagen type IV (atelocollagen)
Purity: Bovine collagen type IV > 90 %; bovine collagen type I and III < 10 %
Purification: Extraction from washed dissected tissue into dilute acetic acid after mild pepsin treatment; purification by differential salt precipitation
Format: Lyophilised, salt-free, sterile (passed sterility test for bacteria and fungi) | Package size: 1 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid; shake or stir for several hours or overnight at 2–8 °C. The material is dissolved when the suspension appears homogeneous with no schlieren pattern. Recommended concentration 1–5 mg/mL. Ships at ambient temperature. Store lyophilised protein 2 years at −20 °C or lower; store reconstituted protein 1 month at +4 °C.
APPLICATIONS
- Cell-culture coating — basement-membrane substrate for endothelial cells, epithelial cells, podocytes, Schwann cells and other type IV-dependent primary cells.
- Adhesion, migration and differentiation assays on a defined basement-membrane matrix.
- ELISA standard, coating antigen and immunogen for anti-collagen type IV antibody work.
- Positive control / reference material for collagen type IV identification and quantification.
- Scaffold and biomaterial functionalisation — basement-membrane component in composite matrices.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Vascular and endothelial biology — basement-membrane coating for angiogenesis and barrier-function models.
- Renal research — glomerular basement-membrane models, Alport and anti-GBM disease studies.
- Tumour invasion — basement-membrane barrier assays and matrix-degradation readouts.
- Neurobiology — Schwann-cell and neurite-outgrowth substrates.
- Antibody and assay development — low-antigenicity atelocollagen IV antigen for immunoassay calibration.
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE.
