148 Collagen type IV (bovine, atelocollagen, sterile, lyophilized)

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Part Number: 148

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Description

Description

Collagen type IV protein purified from bovine placenta (atelocollagen). Sterile and lyophilized

Catalog Number 148

Amount 1 mg

Source

Bovine placenta

Purity

Bovine collagen type IV > 90%. bovine collagen type I and III < 10%

Format

Lyophilized, salt-free

Purification

Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type IV was purified by using differential salt precipitation

Sterility

Passed sterility test for bacteria and fungi

Applications

Cell culture (coating)

Reconstitution

Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4?.

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE

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