Description

Fibronectin 120 kDa fragment containing the cell attachment (RGD) site. Contains interaction sites for integrins alpha5 beta1 and alphaV beta3

Source

Human plasma. Negative for HBsAg, HCV, HIV1 and HIV2

Purity

One major band 120 kDa, no bands higher than 120 kDa

Format

Lyophilized from sodium citrate buffer pH 7.4

Purification

Chymotryptic digest of purified human plasma fibronectin. Affinity chromatography on gelatine agarose, heparin agarose and gel filtration

Applications

Cell attachment assays (promotes efficient adhesion and spreading of cells). Integrin signaling assays (stimulates integrin-dependent activation)

Reconstitution

Sterile pure water 0.5 ml. Incubate 15 min at 37°C. Stir gently. Do not vortex.

Storage

Lyophilized protein: shipping at ambient temperature, storage (1 year) at –20°C or lower. Storage of reconstituted protein 6 months at -70°C. Avoid repeated freeze/thaw cycles