Description
Fibronectin 120 kDa
fragment containing the cell attachment (RGD) site. Contains
interaction sites for integrins alpha5 beta1 and alphaV beta3 Source
Human plasma. Negative for
HBsAg, HCV, HIV1 and HIV2
Purity
One major band 120 kDa, no
bands higher than 120 kDa
Format
Lyophilized from sodium
citrate buffer pH 7.4
Purification
Chymotryptic digest of
purified human plasma fibronectin. Affinity chromatography on
gelatine agarose, heparin agarose and gel filtration
Applications
Cell attachment assays
(promotes efficient adhesion and spreading of cells). Integrin
signaling assays (stimulates integrin-dependent activation)
Reconstitution
Sterile pure water 0.5
ml. Incubate 15 min at 37°C. Stir gently. Do not vortex.
Storage
Lyophilized protein:
shipping at ambient temperature, storage (1 year) at –20°C or
lower. Storage of reconstituted protein 6 months at -70°C. Avoid
repeated freeze/thaw cycles |