Mouse collagen type I purified from tail tendon, native (telopeptide-intact), sterile and lyophilised, 1 mg — suitable for 3D gel formation and cell-culture coating. Acid extraction without pepsin treatment preserves the intact triple helix and its telopeptides, so the molecule retains full native fibrillogenesis: neutralised at 37 °C it self-assembles into a fibrillar hydrogel with native cross-linking competence, the standard matrix for mouse-derived primary cells and syngeneic 3D models. The telopeptide-depleted (low-antigenicity) mouse alternative is cat. 603; see also mouse collagen type I cat. 342 and mouse collagen type I and III cat. 221.
SPECIFICATIONS
Catalogue number: 176 | Source: Mouse tail tendons
Composition: Collagen type I, native (not pepsin-treated)
Purity: Mouse collagen type I > 90 %; mouse collagen type III < 10 %
Purification: Extraction from washed dissected tissue into dilute acetic acid; purification by differential salt precipitation
Format: Lyophilised, salt-free, sterile (passed sterility test for bacteria and fungi) | Package size: 1 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid; shake or stir for several hours or overnight at 2–8 °C. The material is dissolved when the suspension appears homogeneous with no schlieren pattern. Recommended concentration 1–5 mg/mL. Ships at ambient temperature. Store lyophilised protein 2 years at −20 °C or lower; store reconstituted protein 1 month at +4 °C.
APPLICATIONS
- 3D collagen gel / hydrogel formation — native fibrillogenesis on neutralisation at 37 °C for cell encapsulation, spheroid and organoid culture.
- Cell-culture coating substrate for mouse primary cells, fibroblasts, stem cells and organoid lines.
- Contraction, migration and invasion assays in floating or anchored collagen lattices.
- ELISA standard, coating antigen and immunogen for anti-collagen type I antibody work.
- Species-matched reference material for mouse collagen identification and quantification.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Syngeneic mouse 3D models — species-matched ECM for murine tumour, immune and stromal co-cultures.
- Fibrosis research — native collagen I lattices for fibroblast contraction and myofibroblast differentiation assays.
- Organoid and stem-cell culture — gel-forming matrix for murine intestinal, mammary and neural organoids.
- Fibrillogenesis and matrix-biophysics studies — telopeptide-intact collagen for assembly kinetics and stiffness tuning.
- Antibody and assay development — mouse collagen I antigen for immunoassay calibration and cross-reactivity testing.
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE.
