Bovine collagen type V (atelocollagen) purified from placenta, sterile and lyophilised, 1 mg — the minor fibrillar collagen that nucleates and regulates type I fibril diameter, supplied as a cell-culture coating substrate and immunoassay antigen. Mild pepsin treatment removes the telopeptides, lowering cross-species antigenicity while preserving the triple-helical domain. The bovine counterpart of human collagen type V cat. 436; completes the bovine ECM panel with type I cat. 437, type III cat. 576 and type IV cat. 148.
SPECIFICATIONS
Catalogue number: 184 | Source: Bovine placenta
Composition: Collagen type V (atelocollagen)
Purity: Bovine collagen type V > 98 %
Purification: Extraction from washed dissected tissue into dilute acetic acid after mild pepsin treatment; purification by differential salt precipitation
Format: Lyophilised, salt-free, sterile (passed sterility test for bacteria and fungi) | Package size: 1 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid; shake or stir for several hours or overnight at 2–8 °C. The material is dissolved when the suspension appears homogeneous with no schlieren pattern. Recommended concentration 1–5 mg/mL. Ships at ambient temperature. Store lyophilised protein 2 years at −20 °C or lower; store reconstituted protein 1 month at +4 °C.
APPLICATIONS
- Cell-culture coating — substrate for corneal keratocytes, fibroblasts, endothelial and epithelial cells.
- Fibrillogenesis studies — co-assembly with type I collagen to control fibril nucleation and diameter in reconstituted matrices.
- ELISA standard, coating antigen and immunogen for anti-collagen type V antibody work.
- Positive control / reference material for collagen type V identification and quantification.
- Antigen for autoimmunity research — collagen V reactivity in transplant and lung-fibrosis immunology.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Lung-transplant immunology — collagen V is a recognised autoantigen in chronic allograft rejection and bronchiolitis obliterans research.
- Corneal and scleral biology — type V is enriched in corneal stroma and controls fibril thinness.
- Matrix assembly — regulating collagen I fibril diameter in reconstituted 3D gels.
- Connective-tissue disease models — classical Ehlers-Danlos syndrome (COL5A1 / COL5A2) research.
- Antibody and assay development — high-purity type V antigen for immunoassay calibration and cross-reactivity testing.
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE.
