Mouse monoclonal antibody against human CD36 (platelet GPIV / GPIIIb, the thrombospondin receptor; ~90 kDa), clone VM58, 0.2 mg. Reacts with CD36 on platelets, monocytes, macrophages and erythroblasts (weakly on B-cells); in immunohistology it also stains some endothelial cells, adipocytes and the granular layer of the skin. For functional platelet studies, indirect immunofluorescence and immunohistochemistry.
SPECIFICATIONS
Catalogue number: 192 | Host: Mouse | Target: Human
Target antigen: CD36 (platelet GPIV / GPIIIb, thrombospondin receptor; ~90 kDa)
Gene: CD36 (7q21.11) | UniProt: P16671
Clonality: Monoclonal | Clone: VM58 | Conjugate: Unconjugated
Reactivity: Platelets, monocytes, macrophages and erythroblasts; weak on B-cells. IHC: some endothelial cells, adipocytes and the granular layer of the skin.
Purification: Ammonium sulphate precipitation and DEAE-cellulose chromatography.
Format: Lyophilised (mannitol, dextran, salts) | Package size: 0.2 mg
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- Functional studies — platelet CD36 / thrombospondin-receptor function and Fc-receptor-dependent platelet activation.
- Indirect immunofluorescence — on cells; fixation with 1 % paraformaldehyde for 5 minutes is advised.
- IHC — CD36 detection in tissue (endothelium, adipocytes, skin granular layer).
- Note: Western blot is not recommended.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Platelet biology / thrombosis research — CD36 (GPIV) function and platelet activation.
- Monocyte / macrophage and foam-cell biology — CD36 scavenger-receptor studies in atherosclerosis.
- Adipocyte and metabolic research — CD36-mediated fatty-acid uptake.
- Angiogenesis — CD36 as the thrombospondin-1 receptor.
REFERENCES
1) Gardiner EE, Karunakaran D, Arthur JF, Mu FT, Powell MS, Baker RI, Hogarth PM, Kahn ML, Andrews RK, Berndt MC. (2008) Dual ITAM-mediated proteolytic pathways for irreversible inactivation of platelet receptors: de-ITAM-izing Fc?RIIa. Blood. 111(1):165-174.
2) Burgess JK, Lindeman R, Chesterman CN, Chong BH. (1995) Single amino acid mutation of Fc gamma receptor is associated with the development of heparin-induced thrombocytopenia. Br J Haematol. 91(3):761-766.
3) Mazurov AV, Vinogradov DV, Vlasik TN, Burns GF, Berndt MC. (1992) Heterogeneity of platelet Fc-receptor-dependent response to activating monoclonal antibodies. Platelets. 3(4):181-188.
4) Vinogradov DV, Vlasik TN, Agafonova OG, Vasil’ev SA, Lagutina NIa, Makarov VA, Berndt M, Mazurov AV. (1991) Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex. Biokhimiia. 56(5):787-797.
