anti-human CD36 (Platelet GPIV, Thrombospondin receptor) (clone VM58,
IgG1 kappa fraction)
The monoclonal antibody is
directed against the CD36 antigen (GP IV, GP IIIa, Thrombospondin
receptor) (90 kDa), which is expressed on human platelets. Antibody
reacts with platelets, monocytes, macrophages, erythroblasts and
weakly with B-cells. In immunohistology the antibody reacts with some
endothelial cells, adypocytes and the granular layer of the skin.
Antibody induces Fc-receptor-dependent platelet aggregation after
binding to platelet. The heterogeneity of the response found in
healthy individuals is dependent on the polymorphism of the Fc RII on
precipitation and DEAE-cellulose chromatography with gradient elution
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Indirect immunofluorescence. Innumohistochemistry.
For fluorescence technique
on cells fixation with 1% paraformaldehyde for 5 minutes is advised.
Use for WB is not recommended
Use 1 ml sterile pure
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
Gardiner EE, Karunakaran D, Arthur JF, Mu FT, Powell MS, Baker RI, Hogarth PM, Kahn ML, Andrews RK, Berndt MC. (2008) Dual ITAM-mediated proteolytic pathways for irreversible inactivation of platelet receptors: de-ITAM-izing FcgammaRIIa. Blood. 111(1):165-174
Burgess JK, Lindeman R, Chesterman CN, Chong BH. (1995) Single amino acid mutation of Fc gamma receptor is associated with the development of heparin-induced thrombocytopenia. Br J Haematol. 91(3):761-766
Mazurov AV, Vinogradov DV, Vlasik TN, Burns GF, Berndt MC. (1992) Heterogeneity of Platelet Fc-receptor-dependent Response to Activating Monoclonal Antibodies. Platelets. 3(4):181-188
Vinogradov DV, Vlasik TN, Agafonova OG, Vasil'ev SA, Lagutina NIa, Makarov VA, Berndt M, Mazurov AV. (1991) [Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex]. Biokhimiia. 56(5):787-797