Native human collagen type II, isolated from human cartilage by partial pepsin digestion under acidic conditions followed by differential salt precipitation, supplied lyophilised at >95% purity, 0.1 mg. Donor cartilage is screened negative for HBsAg, HCV and HIV-1/HIV-2. Type II is the defining fibrillar collagen of articular cartilage, the vitreous humour and the nucleus pulposus, and the dominant autoantigen in human rheumatoid arthritis and in collagen-induced-arthritis animal models. Human-source type II collagen is difficult to obtain, and a species-matched human standard avoids the cross-species epitope artefacts introduced by bovine, chicken or porcine substitutes. Use as an ELISA / Western blot standard, as immunogen, or as a cell-culture coating substrate — the matching antigen for the anti-collagen type II antibodies cat. 214 and cat. 415, and part of the human-source ECM antigen panel together with cat. 288 (collagen type I), cat. 889 (collagen type III) and cat. 436 (collagen type V). Bovine-source type II is available in bulk as cat. 826-1 and cat. 826-30.
SPECIFICATIONS
Catalogue number: 210 | Product: Collagen type II (human)
Source: human cartilage (donor screened negative for HBsAg, HCV, HIV-1 / HIV-2)
Purity: collagen type II >95%; collagen types I + III <5% combined; other human collagens and non-collagen proteins <0.5%
Purification: partial pepsin digestion under acidic conditions; differential salt precipitation
Format: lyophilised | Package size: 0.1 mg
STORAGE / RECONSTITUTION
Dissolve in 0.5 M acetic acid, pH 2.5. Stir or shake for several hours or overnight at 2–8 °C; the protein is fully dissolved when the solution is homogeneous with no schlieren pattern.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- ELISA, immunoblot and dot-blot reference standard for human collagen type II — pair with cat. 214 or cat. 415 as the detection antibody.
- Immunogen for the production of anti-collagen type II antibodies.
- Coating antigen for anti-collagen type II autoantibody serology on patient sera.
- Cell-culture coating substrate for chondrocyte adhesion, migration and differentiation assays.
- Defined human antigen for in-vitro restimulation of T cells in collagen-induced-arthritis (CIA) protocols and for cross-species reactivity panels.
- Substrate for type-II-specific protease activity assays (MMP-1, MMP-3, MMP-8, MMP-13, cathepsin K).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rheumatoid arthritis autoimmunity: the dominant human autoantigen for anti-CII autoantibody detection and for human-cell-based autoreactivity readouts.
- Species-matched human reagent where bovine, chicken or porcine type II would introduce cross-species epitope artefacts.
- Intervertebral disc research: nucleus pulposus and notochordal matrix biology.
- Cartilage tissue engineering: chondrogenic scaffold and plate coating.
- Single-molecule biophysics: native triple-helical collagen for AFM, optical-tweezers, persistence-length and SAXS / SANS studies.
- Osteoarthritis drug discovery: defined human substrate for cartilage-degrading protease assays.
REFERENCES
Capossela S., Schläfli P., Bertolo A., et al. (2014) Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. European Cells and Materials 27: 251–263.
Pal S., Konkimalla V.B. (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief 8: 1032–1037. doi:10.1016/j.dib.2016.03.105.
Rezaei N., Lyons A., Forde N.R. (2018) Environmentally controlled curvature of single collagen proteins. bioRxiv 279380. doi:10.1101/279380.
Gualandi L., Kamath R., Schwartz Sterman A. (2017) Anti-collagen antibodies for treatment and diagnosis. US patent application US20170226196A1.
