Collagen Type II protein purified from human cartilage
Source
Human cartilage. Negative
for HBsAg, HCV, HIV1 and HIV2
Purity
Human collagen type II >
95%, human collagen types I and III < 5%, other human collagen
types and non-collagen proteins < 0.5%.
Format
Lyophilized
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for immunisation (1) and antibody production.
Coating material for cell culture studies.
Reconstitution
Use 0.5 M acetic
acid, pH 2.5
Storage
Shipping at ambient
temperature. Storage 2 years at -20°C or lower
Citations
1. Gualandi L, Kamath R, Schwartz Sterman A (2014) Anti-collagen antibodies for treatment and diagnosis. US20170226196A1 patent application
2. Pal S, V. Badireenath Konkimalla (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief. https://doi.org/10.1016/j.dib.2016.03.105
3. Rezaei N, Lyons A, Forde NR (2018) Environmentally controlled curvature of single collagen proteins. bioRxiv http://dx.doi.org/10.1101/279380
