Murine collagen type I and III purified from tail tendons of the mouse
Mouse tail tendons
Murine collagens: type I –
45%, type III – 45%, type IV – 10%, type V below 1%. Non-collagen
proteins below 0.5%
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Coating material for cell
culture studies. May not be suitable for 3-D gel formation
Use 0.05-0.5 M acetic
acid, pH 2.5 at 4°C.
Dissolved collagen retains immunologic properties of native collagen.
Structure of native collagen confirmed by ability to form
Shipping at ambient
temperature. Storage of lyophilized protein 2 years at -20°C.
Storage of reconstituted protein 1 month at +4°C