Murine collagen type I and III purified from tail tendons of the mouse

Source

Mouse tail tendons

Purity

Murine collagens: type I – 45%, type III – 45%, type IV – 10%, type V below 1%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.05-0.5 M acetic acid, pH 2.5 at 4°C. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C