Native pentameric human C-reactive protein (CRP / pCRP), purified from human ascitic fluid by calcium-dependent affinity chromatography on CH-Sepharose followed by hydroxylapatite chromatography, supplied as a sterile-filtered 10 mg/mL solution in calcium-stabilised buffer, 1 mg. Donors screened negative for HBsAg, HCV and HIV-1 / HIV-2 antibodies. The calcium-dependent pentameric quaternary structure is preserved and the preparation is free of stabilising additives that interfere with downstream chemistry, so it can be used directly — as an immunogen, an ELISA coating antigen, an affinity ligand, or as the starting material for generating bioactive monomeric CRP (mCRP) by the Potempa urea / EDTA chelation method.
SPECIFICATIONS
Catalogue number: 226 | Product: C-reactive protein (human)
Source: human ascitic fluid (donors HBsAg / HCV / HIV-1 / HIV-2 antibody-negative)
Purity: >98% on reduced SDS-PAGE; single band at 26 kDa
Purification: calcium-dependent affinity chromatography on CH-Sepharose, followed by hydroxylapatite chromatography
Quaternary form: native pentamer (calcium-dependent assembly preserved by the 2 mM CaCl2 formulation)
Format: sterile-filtered solution, 10 mg/mL in 100 mM NaCl, 10 mM Tris, 2 mM CaCl2, 0.1% sodium azide | Package size: 1 mg
STORAGE
Supplied ready to use; no reconstitution required.
Ships at ambient temperature.
Long-term storage: 1 year at +4 °C.
DO NOT FREEZE — freezing disrupts the calcium-dependent pentameric assembly.
APPLICATIONS
- Immunogen for raising polyclonal and monoclonal anti-CRP antibodies.
- ELISA / immunoassay coating antigen for plate-based CRP detection and antibody-titre assays.
- Affinity-chromatography ligand for capture of anti-CRP antibodies, CRP-binding proteins and complement component C1q.
- Starting material for monomeric CRP (mCRP) preparation by the Potempa urea / EDTA chelation method (10 mM EDTA, 8 M urea, 37 °C 2 h, followed by dialysis).
- Standard / calibrator for in-house CRP assay development.
- Substrate for studies of CRP–ligand interactions (phosphocholine, C1q, lipoproteins, Fc receptors).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Neuroinflammation: mCRP derived from this material is an established pro-inflammatory agonist in microglial (BV2, primary glial) models of Alzheimer-related inflammation.
- Additive-free formulation: no BSA, glycerol or sucrose, so the pentamer can be dissociated cleanly to mCRP without interfering species.
- Endothelial-raft and chondrocyte-activation studies using monomeric CRP.
- Cardiovascular-risk biomarker assay development: calibrator for high-sensitivity CRP methods.
- Innate-immunity research: pentameric CRP as a C1q-binding, complement-activating pattern-recognition molecule.
REFERENCES
Bartra C., et al. (2024) Resveratrol activates antioxidant protective mechanisms in cellular models of Alzheimer’s disease inflammation. Antioxidants 13(2): 177. doi:10.3390/antiox13020177.
Bartra C., et al. (2025) Microglial pro-inflammatory mechanisms induced by monomeric C-reactive protein are counteracted by soluble epoxide hydrolase inhibitors. International Immunopharmacology 155: 114644.
Bartra i Cabré C. (2024) The impact of soluble epoxide hydrolase inhibition and epoxyeicosatrienoic acids on early-life microglia activation and neuroinflammatory responses in the brain. PhD thesis, University of Barcelona.
