Bovine collagen type VI, isolated from bovine placenta by pepsin treatment and extraction into dilute acetic acid followed by differential salt precipitation, supplied lyophilised and salt-free at >90% purity, 1 mg. Type VI is the beaded-filament collagen of the pericellular matrix, forming a microfibrillar network that anchors cells — chondrocytes, myofibres, adipocytes — to the surrounding interstitial matrix and binds collagen types I, II and IV, fibronectin, biglycan and decorin. Use as an ELISA / Western blot standard, an immunoprecipitation antigen or a cell-culture coating substrate. Pair with the anti-collagen type VI antibodies cat. 471 and cat. 835 for immunoassay detection.
SPECIFICATIONS
Catalogue number: 231 | Product: Collagen type VI (bovine)
Source: bovine placenta
Purity: bovine collagen type VI >90%
Purification: pepsin treatment and extraction of washed, dissected tissue into dilute acetic acid; purification of collagen type VI by differential salt precipitation
Format: lyophilised, salt-free | Package size: 1 mg
STORAGE / RECONSTITUTION
Collagen type VI dissolves very slowly in 0.5 M acetic acid and requires both vigorous mixing and time. Add a small volume of 0.5 M acetic acid, pH 2.5, and use a Potter homogeniser (or similar) to break the protein cake into suspension. Add further 0.5 M acetic acid, pH 2.5, to a concentration of 0.1 mg/mL and stir vigorously for several days. Concentrations above 0.1 mg/mL require even longer; use 6 M urea (or another chaotropic agent) if the application allows.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- ELISA and Western blot reference standard for collagen type VI — pair with cat. 471 or cat. 835 as the detection antibody.
- Immunoprecipitation antigen and antibody-specificity control.
- Immunogen for the production of anti-collagen type VI antibodies.
- Cell-culture coating substrate for pericellular-matrix adhesion and migration assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Pericellular-matrix biology: the defining beaded-filament collagen of the chondrocyte and myofibre pericellular niche.
- Muscular dystrophy research: collagen VI deficiency underlies Ullrich congenital muscular dystrophy and Bethlem myopathy.
- Adipose-tissue and tumour-microenvironment studies, where collagen VI is a major stromal and adipocyte-derived matrix component.
- Cartilage research: collagen VI as the chondron marker in osteoarthritis models.
- Matrix-interaction studies with collagen I / II / IV, fibronectin, biglycan and decorin.
