Source

Bovine placenta

Purity

Bovine collagen type VI 90%

Format

Lyophilized, salt-free

Purification

Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type VI was purified by using differential salt precipitation

Applications

ELISA, Immunoprecipitation, Western Blotting

Reconstitution

Collagen type VI dissolves very slowly in 0.5 M acetic acid and requires both vigorous mixing and time.

Add small amount of 0.5 M acetic acid, pH 2.5 and use Potter homogenizer (or similar) to break up the protein cake into suspension. Add 0.5 M acetic acid, pH 2.5 to yield 0.1 mg/ml concentration and use vigorous stirring for several days.

Concentration higher than 0.1 mg/ml in acetic acid requires even longer time. Use 6M urea (or other chaotropic agent) if application allows it.

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC