YO Proteins AB Home | My Account | Customer Service | Shopping Cart Shopping Cart - 0 item(s) / Total: $0
Home | My Account | Customer Service | Shopping Cart Shopping Cart - 0 item(s) / Total: $0
primary antibodies
secondary antibodies
proteins
Collagen VI
affinity resins
Bioink

Home>proteins
231 Collagen type IV bovine
Part Number 231
Price
Your Price:
$195.00
Availability:
In Stock
Share |
Quantity
Description

Bovine collagen type VI isolated from placenta


Catalog number 231
Amount 1 mg

Source

Bovine placenta

Purity

Bovine collagen type VI 90%

Format

Lyophilized, salt-free

Purification

Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type VI was purified by using differential salt precipitation

Applications

ELISA, Immunoprecipitation, Western Blotting

Reconstitution

Collagen type VI dissolves very slowly in 0.5 M acetic acid and requires both vigorous mixing and time.

Add small amount of 0.5 M acetic acid, pH 2.5 and use Potter homogenizer (or similar) to break up the protein cake into suspension. Add 0.5 M acetic acid, pH 2.5 to yield 0.1 mg/ml concentration and use vigorous stirring for several days.

Concentration higher than 0.1 mg/ml in acetic acid requires even longer time. Use 6M urea (or other chaotropic agent) if application allows it.

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20C or lower. Storage of reconstituted protein 1 month at +4C


Browse Similar Items
conjugation>unconjugated
protein species>cow (bovine)
protein>Collagen VI