Urokinase (uPA) colorimetric substrate. L-Pyroglutamyl-glycyl-L-arginine-p-Nitroaniline hydrochloride (pyroGlu-Gly-Arg-pNA*HCl). Mw 499

Purity

97% HPLC

Format

Lyophilized. Contains 100 mg of urokinase substrate and 900 mg D-mannitol

Applications

Activity assay. Activity is measured by increase of optical density (405 nm) at 37C at 0.025 min-1 in buffer (0.14M NaCl, 0.01M Na-phosphate pH 7.5, 0.2 mM of substrate and 100 IU/ml of urokinase (cat 282))

Reconstitution

Dissolve in distilled water.

Storage

Shipping at ambient temperature. Lyophilized product is stored for 2 years at -20°C, dry and dark. Reconstituted product is kept at +4°C for 2 months