Goat polyclonal antibody against the human IgM heavy (µ) chain, affinity-purified, 1 mg. A single, well-characterised anti-human-IgM reagent for isotype-specific IgM ELISA (detection or capture), immunoblot and IgM affinity purification — cited as the IgM-specific detection reagent (1:10 000) in anti-SARS-CoV-2 Spike-RBD serology ELISAs. Unconjugated format suitable for custom HRP / FITC / AP / biotin conjugation. The single-isotype complement to the pan-class cat. 172 (rabbit anti-human IgG + IgM + IgA).
SPECIFICATIONS
Catalogue number: 321 | Host: Goat | Target: Human
Target antigen: Human IgM heavy (µ) chain
Reactivity: Human IgM (µ-chain)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Human IgM µ-chain specific.
Purification: Affinity-purified.
Format: Unconjugated, affinity-purified | Package size: 1 mg | Recommended ELISA detection dilution (after HRP conjugation): 1:10 000
STORAGE
Ships at ambient temperature. Store at -20 °C or lower; aliquot to avoid repeated freeze-thaw. Avoid microbial contamination.
APPLICATIONS
- ELISA — IgM-specific detection antibody (after conjugation) or capture antibody for IgM sandwich ELISA (~1:10 000).
- Western blot / immunoblot — detection of the human IgM heavy chain.
- Affinity purification — column ligand for purification or depletion of human IgM from serum.
- Custom conjugation — HRP, FITC, AP, biotin or fluorophores for in-house detection reagents.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Infectious-disease serology — IgM-specific antibody titres (e.g. anti-SARS-CoV-2 Spike-RBD IgM).
- Acute-infection / seroconversion research — IgM as the early-response isotype.
- IgM sandwich-ELISA assay development.
- IgM affinity purification / depletion.
REFERENCES
1) Schlesinger T, et al. (2020) Biodistribution and serologic response in SARS-CoV-2 induced ARDS: a cohort study. PLOS ONE 15(11):e0242917. doi:10.1371/journal.pone.0242917. — cat. 321 used at 1:10 000 as the IgM-specific detection reagent in an anti-SARS-CoV-2 Spike-RBD ELISA.
2) Notz Q, et al. (2020) Pro- and anti-inflammatory responses in severe COVID-19-induced acute respiratory distress syndrome — an observational pilot study. Front Immunol. 11:581338. doi:10.3389/fimmu.2020.581338. — an HRP-conjugated YO anti-human IgM used at 1:10 000 as the IgM detection reagent.
