Description
Goat-host polyclonal antibody directed against human IgM µ heavy chain. Goat is the most widely used host for human-serology secondary reagents because (i) goat antisera pair cleanly with rabbit and mouse primaries in multi-colour stainings, (ii) goat IgG separates well on commercial protein-G columns, and (iii) clinical-diagnostic and reference-method protocols already specify goat as the host of choice for IgG/IgM/IgA isotype-specific detection. The product is supplied in the unconjugated form (cat. 321) so the user can either use it directly as a capture / primary reagent, or conjugate in-house to HRP, FITC, biotin, AP or a fluorophore of choice.
This is the workhorse SKU when a researcher wants a single, well-characterised goat anti-human IgM antibody that can be split across both ELISA detection and immunoblot capture in the same experiment. For ready-conjugated formats, see the HRP- and FITC-conjugated goat anti-human IgM siblings; for monoclonal alternatives, see cat. 445 (mouse clone ICL-931).
Specifications
Catalog number: 321
Amount: 1 mg
Host: Goat
Format: Polyclonal, unconjugated, affinity-purified
Specificity: Human IgM (µ heavy chain)
Recommended dilution: ELISA detection (after HRP conjugation): 1:10,000, Western blot / immunoblot capture: 1:1,000 to 1:5,000 (titrate for each application)
Applications
- ELISA detection antibody for human IgM (after conjugation or in capture format).
- Capture antibody for IgM-specific sandwich ELISA.
- Immunoblot detection of human IgM heavy chain.
- Affinity-column ligand for purification or depletion of human IgM from serum.
- Custom conjugation to HRP, FITC, AP, biotin or fluorophores for in-house assay development.
CITED USE CASES
(1) Schlesinger T. et al. (2020) — Biodistribution and serologic response in SARS-CoV-2 induced ARDS: a cohort study. PLOS ONE 15(11): e0242917. doi:10.1371/journal.pone.0242917.
The authors used the YO Proteins goat anti-human IgM antibody (cat. 321; antibodies-online ID ABIN301951) at 1:10,000 dilution as the IgM isotype-specific detection reagent in an in-house anti-SARS-CoV-2 Spike-RBD ELISA, alongside anti-human IgG (Thermo Fisher 31410) and anti-human IgA (KPL International 5220-0360). RBD was immobilised on NUNC Maxisorp plates, blocked with Roti-Block, detected with TMB substrate and read at 450/620 nm on a Tecan Sunrise. The validated assay was applied to serum and tissue compartments of COVID-19 ARDS patients to map systemic and bronchoalveolar IgM responses against SARS-CoV-2.
(2) Notz Q. et al. (2020) — Pro- and anti-inflammatory responses in severe COVID-19-induced acute respiratory distress syndrome — an observational pilot study. Frontiers in Immunology 11: 581338. doi:10.3389/fimmu.2020.581338.
Notz et al. used a horseradish-peroxidase-conjugated anti-human IgM antibody from YO Proteins (Rönninge, Sweden) at 1:10,000 dilution as the IgM detection reagent in a parallel anti-SARS-CoV-2 Spike-RBD ELISA on COVID-19 ARDS patient serum. The reagent quantified anti-Spike-RBD IgM levels down to =4 ng/mL, alongside isotype-specific IgG (Thermo Scientific) and IgA (International Limited, New Delhi) detection. Together with Schlesinger 2020 this establishes the YO Proteins goat anti-human IgM polyclonal as a validated detection antibody in published COVID-19 serology.
