Rat collagen types I and III, co-purified and supplied lyophilised and salt-free, 10 mg. The preparation contains both of the fibrillar collagens that dominate rat connective tissue, in a 70:30 type I : type III ratio, and is intended as a species-matched immunoassay standard, immunogen and cell-culture coating substrate for rodent work. Complementary reagents: single-type rat collagen I cat. 351; the murine counterpart cat. 221 (mouse collagen I + III); the detection antibodies cat. 352 (anti-rat collagen I + III) and cat. 376 (anti-rat collagen I); and, for 3D gel work, the rat atelocollagen cat. 602.
SPECIFICATIONS
Catalogue number: 362 | Product: Collagen type I and III (rat)
Source: rat
Purity: rat collagen type I 70%; rat collagen type III 30%; other rat collagen types <1%; non-collagen proteins <0.5%
Format: lyophilised, salt-free | Package size: 10 mg
STORAGE / RECONSTITUTION
Dissolve in 0.5 M acetic acid, pH 3.0.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower.
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Rat collagen type I / III ELISA, immunoblot and dot-blot reference standard — pair with cat. 352 as the detection antibody.
- Species-matched immunogen for anti-rat collagen antibody production and cross-reactivity testing.
- Cell-culture coating substrate for rat fibroblast and connective-tissue adhesion, migration and proliferation assays.
- Substrate for matrix metalloproteinase (MMP) activity assays on rodent collagen.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Fibrosis and wound-healing models: the type I : type III ratio is the classic readout of scar maturation, and both types are present together in this preparation.
- Species matching for rat models: avoids cross-species epitope artefacts introduced by bovine or human collagen.
- Antibody development: native-conformation rat immunogen for antibodies intended for use on rat tissue.
- Rodent ECM-remodelling and tissue-engineering studies.
- Cross-species reactivity panels alongside the mouse (cat. 221) and rat type I (cat. 351) collagens.
