Goat polyclonal antibody against human collagen type V, affinity-purified, 1 mL. Recognises native human collagen V with no cross-reactivity to human plasma proteins or to human collagen types I, II, III or IV. A type-V-specific reagent for sandwich ELISA and frozen-section histochemistry: pair with the mouse monoclonal cat. 488 (clone MC5-HA) on the opposing arm and use cat. 436 (human collagen type V antigen) as the standard; complements the affinity-purified rabbit polyclonal cat. 201.
SPECIFICATIONS
Catalogue number: 417 | Host: Goat | Target: Human
Target antigen: Native human collagen type V
Gene family: COL5 (COL5A1 / COL5A2 / COL5A3)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Recognises native human collagen type V; no cross-reactivity to human plasma proteins or to human collagen types I, II, III or IV. Native-conformation — not suitable for denaturing or dissociating conditions.
Purification: Affinity-purified, with cross-absorption against immobilised human plasma proteins and human collagen types I–IV
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C for up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — anti-collagen-V detection / capture arm (dilution ×3000). Pair with the mouse monoclonal cat. 488 (clone MC5-HA); use cat. 436 (human collagen type V antigen) as the standard.
- Histochemistry (frozen sections) — human collagen-V localisation (dilution ×20).
- Type-V specificity — no cross-reactivity to collagen I–IV or to human plasma proteins.
- Note: native-conformation antibody — not suitable for denaturing or dissociating conditions (e.g. reducing Western blot).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Ehlers–Danlos syndrome (classical type) research — collagen V is the mutated collagen in classical EDS (COL5A1 / COL5A2).
- Fibril architecture — collagen V nucleates and regulates the diameter of type-I collagen fibrils in connective tissue.
- Corneal biology — collagen V contributes to corneal stromal transparency and lamellar organisation.
- Vascular & placental matrix — collagen V in blood-vessel walls and perivascular extracellular matrix.
- Tumour stroma & fibrosis — collagen-V deposition in desmoplastic and fibrotic tissue.
