Bovine collagen type I (atelocollagen), purified from bovine tendon by mild pepsin treatment, extraction into dilute acetic acid and differential salt precipitation, supplied sterile and lyophilised, salt-free, 30 mg. The extended pepsin step removes the immunogenic non-helical telopeptides, yielding an atelocollagen that retains its triple-helical structure and self-assembly behaviour: reconstituted material gels at neutral pH and 37 °C and is therefore suitable both as a 3D scaffold matrix and as a 2D culture-surface coating. It is the type I partner to the bovine type III atelocollagen cat. 576 for building collagen I/III scaffolds, complements the non-sterile bovine collagen type I preparation cat. 631, and pairs with the anti-bovine collagen type I and III antibody cat. 389.
SPECIFICATIONS
Catalogue number: 437 | Product: Collagen type I (bovine, atelocollagen)
Source: bovine tendon
Purity: bovine collagen type I 95%
Purification: mild pepsin treatment of washed dissected tissue, extraction into dilute acetic acid, and differential salt precipitation
Sterility: passed sterility testing for bacteria and fungi
Format: lyophilized, salt-free, sterile
Package size: 30 mg
STORAGE / RECONSTITUTION
Dissolve in 10–50 mM sterile acetic acid. Stir the suspension gently for several hours or overnight at +4 °C. The protein is fully dissolved when the solution appears homogeneous with no schlieren pattern (visible light refraction caused by differing protein concentrations).
Recommended working concentration: 1–5 mg/mL.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- 3D cell culture: collagen gels and hydrogels formed by neutralisation and incubation at 37 °C.
- 2D cell-culture coating substrate for adhesion, migration and proliferation assays.
- Tissue-engineering and bioprinting scaffolds; blend with the bovine type III atelocollagen cat. 576 to reproduce physiological collagen I/III ratios.
- Protein standard for collagen type I ELISA and Western blot — pair with cat. 389 as the detection antibody.
- Substrate for matrix metalloproteinase (MMP) activity assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Sterile, bulk-format (30 mg) atelocollagen for scaling 3D organoid and spheroid culture without additional sterilisation steps.
- Low-immunogenicity scaffold material for tissue-engineering constructs and regenerative-medicine models.
- Defined-stiffness hydrogels for mechanotransduction and durotaxis studies (gel modulus tuned by collagen concentration).
- Tumour-invasion and cell-migration assays in 3D collagen matrices.
- Bioink component for extrusion bioprinting of ECM-mimetic constructs.
- Dermal-equivalent and vascular scaffold models when blended with type III collagen.
