Rabbit polyclonal antibody against human collagen type VI, pre-adsorbed, ~4 mg IgG, 1 mL. Reacts with both native and heat-denatured human collagen VI — in competitive ELISA collagen VI = 100%, collagen I/III = 5%, collagen IV/V = 10%, with no interference from human plasma proteins. Suitable for native and denaturing workflows; use with the affinity-purified sibling cat. 835 (rabbit anti-human collagen VI) and the collagen VI antigen cat. 231 as the ELISA standard / immunogen.
SPECIFICATIONS
Catalogue number: 471 | Host: Rabbit | Target: Human
Target antigen: Human collagen type VI
Gene family: COL6 (COL6A1 / COL6A2 / COL6A3)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human collagen type VI (native and heat-denatured); competitive-ELISA cross-reactivity collagen I/III 5%, collagen IV/V 10%. Human plasma proteins do not interfere.
Purification: Pre-adsorption against human plasma proteins and collagen types I, III, IV and V; sulphate precipitation and ion-exchange chromatography
Format: Lyophilised rabbit IgG (~4 mg) from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C for up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA (dilution x4000) — quantification of human collagen type VI; use antigen cat. 231 as the standard.
- Immunohistochemistry (dilution x20) — collagen VI localisation in tissue (native and denatured epitopes).
- Western blot — reacts with heat-denatured collagen VI.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Muscular dystrophy research — COL6 mutations cause Bethlem myopathy and Ullrich congenital muscular dystrophy.
- Extracellular matrix biology — collagen VI microfibril networks linking cells to the ECM.
- Fibrosis — collagen VI deposition in skeletal muscle, adipose and organ fibrosis.
- Tumour microenvironment — collagen VI signalling in cancer-associated stroma and adipocytes.
- Regenerative medicine — collagen VI in stem-cell niches and matrix scaffolds.
