Mouse monoclonal antibody against Taq DNA polymerase from Thermus aquaticus, supplied as a 10 mg/mL solution, 100 mg. The antibody binds and inhibits Taq polymerase at ambient temperature and is released on heat denaturation during the initial PCR denaturation step — the classic hot-start PCR mechanism, which suppresses primer-dimer formation and non-specific amplification. A high-purity (> 97 % IgG1), DNA- and RNA-free preparation intended for research use or further manufacturing of hot-start PCR and qPCR master mixes.
SPECIFICATIONS
Catalogue number: 562-100 | Host: Mouse | Target: Taq DNA polymerase (Thermus aquaticus)
Target antigen: Taq DNA polymerase
Clonality: Monoclonal | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Purity: Murine immunoglobulin IgG1 > 97 %; DNA not detectable; RNA not detectable
Activity: Inhibits Taq polymerase activity at a ratio of approximately 0.8 µg antibody per unit of Taq polymerase; inhibition is reversed on heat denaturation.
Format: Solution, 10 mg/mL in 20 mM KCl, 10 mM Tris pH 8.0 | Package size: 100 mg
STORAGE
Ships at ambient temperature. Store up to 2 years at -20 °C or lower. Aliquot to avoid repeated freeze-thaw.
APPLICATIONS
- Hot-start PCR — inhibits Taq at ambient temperature (~0.8 µg antibody per unit of Taq); inhibition is released during the initial 95 °C denaturation step.
- Hot-start master-mix manufacturing — DNA- and RNA-free, bulk 100 mg format for further manufacturing.
- qPCR and diagnostic PCR — reduces primer-dimer and non-specific amplification, improving sensitivity and specificity.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Hot-start PCR / qPCR master-mix production at manufacturing scale.
- Molecular-diagnostic PCR assays where specificity and low background are critical.
- Multiplex PCR — suppression of primer-dimer artefacts.
- DNA/RNA-free grade for nucleic-acid-sensitive workflows.
