Monoclonal mouse antibody anti-taq polymerase 100 mg
Monoclonal antibody anti-taq DNA polymerase from Thermus aquaticus. The primary use of anti-Taq antibody is in "hot start" PCR. The antibody binds to Taq polymerase, inhibiting its activity at room temperature. When the PCR reaction is heated to denaturation temperatures (typically 94–98°C), the antibody denatures, releasing the Taq polymerase and allowing it to begin amplification. This reduces non-specific amplification that can occur at lower temperatures.
Catalog Number 562-100 Quantity 100 mg
Purity
Murine immunoglobulins IgG1 (97%). DNA not detectable. RNA not detectable
Format
Solution 10 mg/ml antibody, 20 mM KCl, 10 mM Tris pH 8
Applications and recommended dilutions
Hot-start PCR. Antibody inhibits activity of taq polymerase in ratio of 0.8 ug of antibody to 1 unit of taq polymerase. Antibody is inactivated at 7-10 min at 95°C
Storage
Shipping at ambient temperature. Storage 2 years -20°C
FOR RESEARCH USE OR FURTHER MANUFACTURING
