Native-conformation bovine collagen type III (atelocollagen), purified from bovine placenta by mild pepsin digestion followed by differential salt precipitation, supplied sterile and lyophilised, salt-free, 10 mg. The extended pepsin step removes the immunogenic non-helical telopeptides, yielding an atelocollagen that retains its triple-helical structure, biological activity and self-assembly behaviour while minimising the cross-species immune response typical of unprocessed collagen. Reconstituted material gels at neutral pH and 37 °C, making it suitable both as a 3D scaffold matrix and as a 2D culture-surface coating. Cat. 576 is the bovine type III counterpart to the bovine type I atelocollagen cat. 437, and is most commonly used as the type III blending component in collagen I/III scaffolds that mimic dermal, vascular or scar-tissue ECM composition, where the type I/III ratio is biologically critical. It complements the non-sterile bovine collagen type III preparation cat. 169 and pairs with the anti-collagen type III antibodies cat. 153 and cat. 380.
SPECIFICATIONS
Catalogue number: 576 | Product: Collagen type III (bovine, atelocollagen)
Source: bovine placenta
Purity: bovine collagen type III above 90%; bovine collagen type I below 10%
Purification: mild pepsin treatment of washed dissected tissue, extraction into dilute acetic acid, and differential salt precipitation
Sterility: passed sterility testing for bacteria and fungi
Format: lyophilized, salt-free, sterile
Package size: 10 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid. Shake or stir between 2–8 °C for several hours, or overnight, until the suspension appears homogeneous with no schlieren pattern (the optical refraction caused by uneven local protein concentration).
Recommended working concentration: 1–5 mg/mL.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- 3D cell culture: collagen gels and hydrogels formed by neutralisation and incubation at 37 °C.
- 2D culture-surface coating for adhesion, migration and proliferation assays.
- Dermal-equivalent, vascular and scar-tissue scaffolds — blend with the bovine type I atelocollagen cat. 437 to reproduce physiological collagen I/III ratios.
- Tissue-engineering and bioprinting workflows requiring a sterile, low-immunogenicity type III component.
- Protein standard for collagen type III ELISA and Western blot — pair with cat. 153 or cat. 380 as the detection antibody.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Defined collagen I/III ratio scaffolds: the type III fraction governs fibril diameter and matrix compliance, a key variable in dermal and vascular tissue engineering.
- Wound-healing and scar-biology models, where the type III to type I transition marks granulation-tissue maturation.
- Vascular ECM and aneurysm research (type III collagen deficiency underlies vascular Ehlers-Danlos syndrome).
- Fibrosis and ECM remodelling assays, including MMP substrate studies.
- Mechanotransduction studies on hydrogels of tunable stiffness and fibril architecture.
- Bioink component for extrusion bioprinting of ECM-mimetic dermal constructs.
