Rat collagen type I (atelocollagen), purified from rat tail tendon by mild pepsin treatment, extraction into dilute acetic acid and differential salt precipitation, supplied sterile and lyophilised, salt-free, 30 mg. Pepsin treatment removes the non-helical telopeptide regions, lowering immunogenicity while preserving the intact triple helix and self-assembly behaviour: reconstituted material gels at neutral pH and 37 °C, making it suitable both as a 3D gel / hydrogel scaffold and as a 2D culture-surface coating. Rat tail collagen type I is the classical substrate for 3D collagen matrices. It complements the non-sterile rat collagen type I preparation cat. 351, and pairs with the anti-rat collagen type I antibody cat. 376 and the anti-rat collagen type I and III antibody cat. 352.
SPECIFICATIONS
Catalogue number: 602 | Product: Collagen type I (rat, atelocollagen)
Source: rat tail tendon
Purity: rat collagen type I above 90%; rat collagen type III below 10%
Purification: mild pepsin treatment of washed dissected tissue, extraction into dilute acetic acid, and differential salt precipitation
Sterility: passed sterility testing for bacteria and fungi
Format: lyophilized, salt-free, sterile
Package size: 30 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid. Shake or stir for several hours or overnight between 2–8 °C. The protein is fully dissolved when the suspension appears homogeneous with no schlieren pattern (light refraction caused by differing protein concentrations).
Recommended working concentration: 1–5 mg/mL.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- 3D cell culture: rat tail collagen gels and hydrogels formed by neutralisation and incubation at 37 °C.
- 2D cell-culture coating substrate for adhesion, migration and proliferation assays.
- Species-matched matrix for rat primary-cell culture (hepatocytes, cardiomyocytes, neurons, fibroblasts).
- Protein standard for rat collagen type I ELISA and Western blot — pair with cat. 376 or cat. 352 as the detection antibody.
- Substrate for matrix metalloproteinase (MMP) activity assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Bulk-format (30 mg) sterile rat tail collagen for scaling 3D organoid, spheroid and tumour-invasion assays without additional sterilisation.
- Rat-model concordance: species-matched matrix for in vitro work supporting rat in vivo studies.
- Contractile collagen-gel assays for fibroblast-mediated matrix remodelling and myofibroblast activation.
- Defined-stiffness hydrogels for mechanotransduction and durotaxis research.
- Primary hepatocyte sandwich culture and neuronal outgrowth substrates.
- Bioink component for extrusion bioprinting of ECM-mimetic constructs.
