Description
Collagen type I protein purified from rat tail tendon (atelocollagen suitable for making 3D gel). Sterile and lyophilized
Catalog Number 602
Amount 30 mg
Source
Rat tail tendons
Purity
Rat collagen type I > 90%, rat collagen type III < 10%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating or 3D gel)
Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4?.
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC
Citations
1) Iizuka S et al (2020) Crosstalk between invadopodia and the extracellular matrix. bioRxiv preprint doi: https://doi.org/10.1101/2020.02.26.966762
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD
