Mouse collagen type I (atelocollagen), purified from mouse tail tendon by mild pepsin treatment, extraction into dilute acetic acid and differential salt precipitation, supplied sterile and lyophilised, salt-free, 1 mg. Pepsin treatment removes the non-helical telopeptide regions, lowering immunogenicity while preserving the intact triple helix and self-assembly behaviour: reconstituted material gels at neutral pH and 37 °C and is therefore suitable for 3D gel / hydrogel work as well as 2D surface coating. This is the sterile, gel-forming murine format; for a pepsin-solubilised coating-grade preparation see cat. 342. It complements the murine collagen type I and III preparation cat. 221 and pairs with the anti-collagen type I antibodies cat. 287 and cat. 393.
SPECIFICATIONS
Catalogue number: 603 | Product: Collagen type I (mouse, atelocollagen)
Source: mouse tail tendon
Purity: mouse collagen type I above 90%; mouse collagen type III below 10%
Purification: mild pepsin treatment of washed dissected tissue, extraction into dilute acetic acid, and differential salt precipitation
Sterility: passed sterility testing for bacteria and fungi
Format: lyophilized, salt-free, sterile
Package size: 1 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid. Shake or stir for several hours or overnight between 2–8 °C. The protein is fully dissolved when the suspension appears homogeneous with no schlieren pattern (light refraction caused by differing protein concentrations).
Recommended working concentration: 1–5 mg/mL.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- 3D cell culture: murine collagen gels and hydrogels formed by neutralisation and incubation at 37 °C.
- 2D cell-culture coating substrate for adhesion, migration and proliferation assays.
- Species-matched matrix for mouse primary-cell and organoid culture.
- Protein standard for collagen type I ELISA and Western blot — pair with cat. 287 or cat. 393 as the detection antibody.
- Substrate for matrix metalloproteinase (MMP) activity assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Murine-model concordance: species-matched, gel-forming matrix for in vitro assays supporting mouse in vivo studies, removing xenogeneic collagen as a confounding variable.
- Mouse organoid and spheroid embedding where a defined, sterile single-component matrix is preferred over undefined basement-membrane extracts.
- Tumour-invasion and cell-migration assays in 3D murine collagen matrices.
- Fibroblast-mediated gel-contraction assays and myofibroblast activation studies.
- Mechanotransduction research on hydrogels of tunable stiffness.
- Immuno-oncology co-culture models requiring syngeneic murine ECM.
